knockout serum replacement medium (Thermo Fisher)
Structured Review

Knockout Serum Replacement Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/knockout+serum+replacement+medium/knockout+serum+replacement+medium/pmc11906159-56-2-7
Average 90 stars, based on 1 article reviews
Images
1) Product Images from "GATA6 regulates WNT and BMP programs to pattern precardiac mesoderm during the earliest stages of human cardiogenesis"
Article Title: GATA6 regulates WNT and BMP programs to pattern precardiac mesoderm during the earliest stages of human cardiogenesis
Journal: eLife
doi: 10.7554/eLife.100797
Figure Legend Snippet:
Techniques Used: Binding Assay, Mutagenesis, Transfection, Construct, CRISPR, Plasmid Preparation, Derivative Assay, Expressing, Control, Sequencing, Recombinant, T7EI Assay, DNA Extraction, DNA Purification, cDNA Synthesis, SYBR Green Assay, Illumina Sequencing, Multiplex Assay, Bicinchoninic Acid Protein Assay, Western Blot, Selection, Software, Knock-Out, Lysis, Electron Microscopy
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Knock-Out:Article Title: Optimization of Buffalo ( Bubalus bubalis ) Embryonic Stem Cell Culture System Article Snippet: .. Briefly the inner cell mass from the embryos was dissected out and seeded overmitomycin-C treated buffalo fetal fibroblast cells in ES medium consisting of Knockout Dulbecco’s Modified Eagle Medium (KO-DMEM, GIBCO/BRL) supplemented with 15% Article Title: DMSO-Free Programmed Cryopreservation of Fully Dissociated and Adherent Human Induced Pluripotent Stem Cells Article Snippet: .. Briefly, cells were cultured in Knockout Dulbecco's modified Eagle's medium (KODMEM, Invitrogen, no. 10829-018) supplemented with 1 mM L-glutamine with 20% Article Title: GATA6 regulates WNT and BMP programs to pattern precardiac mesoderm during the earliest stages of human cardiogenesis Article Snippet: .. Other , Article Title: Directed differentiation of human pluripotent stem cells into epidermal keratinocyte-like cells. Article Snippet: .. 1 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Mouse anti-cytokeratin 14 Thermo Fisher Scientific MAS-11599 Rabbit anti-cytokeratin 18 Abcam Ab181597 Mouse anti-cytokeratin 19 Chemicon CBL198 Rabbit anti-Involucrin Abcam Ab53112 Rabbiti anti-Loricrin Abcam Ab85679 Rabbit anti -E-Cadherin Cell Signaling 24E10 Rabbit anti-Filaggrin Abcam Ab81468 Rabbit anti-Laminin Abcam Ab14509 Mouse anti-Actin Santa Cruz Sc-47778 Donkey anti-rabbit, Alexa Fluor 488 (1:500 dilution) Invitrogen A21206 Goat anti-mouse, Alexa Fluor 488 (1:500 dilution) Invitrogen A21202 Donkey anti-rabbit, Alexa Fluor 594 (1:500 dilution) Invitrogen A10042 Donkey anti-mouse, Alexa Fluor 594 (1:500 dilution) Invitrogen A10037 Chemicals, peptides, and recombinant proteins DMEM/F12 Thermo Fisher Scientific Cat#1260012 Article Title: Directed differentiation of human pluripotent stem cells into epidermal keratinocyte-like cells Article Snippet: DMEM/F12 , Thermo Fisher Scientific , Cat#1260012. .. Article Title: Activation and Inhibition of The Wnt3A Signaling Pathway in Buffalo ( Bubalus bubalis ) Embryonic Stem Cells: Effects of WNT3A, Bio and Dkk1 Article Snippet: .. Briefly, we mechanically dissected the inner cell mass from the embryos under a zoom stereomicroscope and seeded them onto a mitomycintreated buffalo fetal fibroblast feeder layer in ES medium that consisted of knockout Dulbecco’s modified eagle medium ( KO-DMEM, Gibco/ BRL ) supplemented with 15% Article Title: Neural progenitor cells derived from fibroblasts induced by small molecule compounds under hypoxia for treatment of Parkinson's disease in rats. Article Snippet: Rat NSCs were derived from E16 mouse embryos and expanded in a neural expansion medium (NEM; NeuroCult Basal medium, STEMCELL Technologies, Vancouver, BC, Canada), supplemented with 2% B27, 20 ng/mL epidermal growth factor (EGF), and 20 ng/mL basic fibroblast growth factor (bFGF). .. 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Briefly the inner cell mass from the embryos was dissected out and seeded overmitomycin-C treated buffalo fetal fibroblast cells in ES medium consisting of Knockout Dulbecco’s Modified Eagle Medium (KO-DMEM, GIBCO/BRL) supplemented with 15% Article Title: DMSO-Free Programmed Cryopreservation of Fully Dissociated and Adherent Human Induced Pluripotent Stem Cells Article Snippet: .. Briefly, cells were cultured in Knockout Dulbecco's modified Eagle's medium (KODMEM, Invitrogen, no. 10829-018) supplemented with 1 mM L-glutamine with 20% Article Title: Activation and Inhibition of The Wnt3A Signaling Pathway in Buffalo ( Bubalus bubalis ) Embryonic Stem Cells: Effects of WNT3A, Bio and Dkk1 Article Snippet: .. 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Briefly the inner cell mass from the embryos was dissected out and seeded overmitomycin-C treated buffalo fetal fibroblast cells in ES medium consisting of Knockout Dulbecco’s Modified Eagle Medium (KO-DMEM, GIBCO/BRL) supplemented with 15% Article Title: Activation and Inhibition of The Wnt3A Signaling Pathway in Buffalo ( Bubalus bubalis ) Embryonic Stem Cells: Effects of WNT3A, Bio and Dkk1 Article Snippet: .. Briefly, we mechanically dissected the inner cell mass from the embryos under a zoom stereomicroscope and seeded them onto a mitomycintreated buffalo fetal fibroblast feeder layer in ES medium that consisted of knockout Dulbecco’s modified eagle medium ( KO-DMEM, Gibco/ BRL ) supplemented with 15% Cell Culture:Article Title: DMSO-Free Programmed Cryopreservation of Fully Dissociated and Adherent Human Induced Pluripotent Stem Cells Article Snippet: .. Briefly, cells were cultured in Knockout Dulbecco's modified Eagle's medium (KODMEM, Invitrogen, no. 10829-018) supplemented with 1 mM L-glutamine with 20% Article Title: Neural progenitor cells derived from fibroblasts induced by small molecule compounds under hypoxia for treatment of Parkinson's disease in rats. Article Snippet: Rat NSCs were derived from E16 mouse embryos and expanded in a neural expansion medium (NEM; NeuroCult Basal medium, STEMCELL Technologies, Vancouver, BC, Canada), supplemented with 2% B27, 20 ng/mL epidermal growth factor (EGF), and 20 ng/mL basic fibroblast growth factor (bFGF). .. To chemically induce the conversion of REFs into ciNPCs, the initial REFs at P3 cultured in H-DMEM supplemented with 10% fetal bovine serum for 48 hours were transferred into a Article Title: Lentiviral Vectors and Protocols for Creation of Stable hESC Lines for Fluorescent Tracking and Drug Resistance Selection of Cardiomyocytes Article Snippet: .. 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Article Snippet: .. 1 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Mouse anti-cytokeratin 14 Thermo Fisher Scientific MAS-11599 Rabbit anti-cytokeratin 18 Abcam Ab181597 Mouse anti-cytokeratin 19 Chemicon CBL198 Rabbit anti-Involucrin Abcam Ab53112 Rabbiti anti-Loricrin Abcam Ab85679 Rabbit anti -E-Cadherin Cell Signaling 24E10 Rabbit anti-Filaggrin Abcam Ab81468 Rabbit anti-Laminin Abcam Ab14509 Mouse anti-Actin Santa Cruz Sc-47778 Donkey anti-rabbit, Alexa Fluor 488 (1:500 dilution) Invitrogen A21206 Goat anti-mouse, Alexa Fluor 488 (1:500 dilution) Invitrogen A21202 Donkey anti-rabbit, Alexa Fluor 594 (1:500 dilution) Invitrogen A10042 Donkey anti-mouse, Alexa Fluor 594 (1:500 dilution) Invitrogen A10037 Chemicals, peptides, and recombinant proteins DMEM/F12 Thermo Fisher Scientific Cat#1260012 Generated:Article Title: Directed differentiation of human pluripotent stem cells into epidermal keratinocyte-like cells. Article Snippet: .. 1 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Mouse anti-cytokeratin 14 Thermo Fisher Scientific MAS-11599 Rabbit anti-cytokeratin 18 Abcam Ab181597 Mouse anti-cytokeratin 19 Chemicon CBL198 Rabbit anti-Involucrin Abcam Ab53112 Rabbiti anti-Loricrin Abcam Ab85679 Rabbit anti -E-Cadherin Cell Signaling 24E10 Rabbit anti-Filaggrin Abcam Ab81468 Rabbit anti-Laminin Abcam Ab14509 Mouse anti-Actin Santa Cruz Sc-47778 Donkey anti-rabbit, Alexa Fluor 488 (1:500 dilution) Invitrogen A21206 Goat anti-mouse, Alexa Fluor 488 (1:500 dilution) Invitrogen A21202 Donkey anti-rabbit, Alexa Fluor 594 (1:500 dilution) Invitrogen A10042 Donkey anti-mouse, Alexa Fluor 594 (1:500 dilution) Invitrogen A10037 Chemicals, peptides, and recombinant proteins DMEM/F12 Thermo Fisher Scientific Cat#1260012 Isolation:Article Title: Lentiviral Vectors and Protocols for Creation of Stable hESC Lines for Fluorescent Tracking and Drug Resistance Selection of Cardiomyocytes Article Snippet: .. Briefly, cells were cultured in Knockout Dulbecco's modified Eagle's medium (KODMEM, Invitrogen, 10829-018) supplemented with 1 mM L-glutamine with 20% |