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knockout serum replacement medium  (Thermo Fisher)


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    Structured Review

    Thermo Fisher knockout serum replacement medium

    Knockout Serum Replacement Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/knockout+serum+replacement+medium/knockout+serum+replacement+medium/pmc11906159-56-2-7
    Average 90 stars, based on 1 article reviews
    knockout serum replacement medium - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "GATA6 regulates WNT and BMP programs to pattern precardiac mesoderm during the earliest stages of human cardiogenesis"

    Article Title: GATA6 regulates WNT and BMP programs to pattern precardiac mesoderm during the earliest stages of human cardiogenesis

    Journal: eLife

    doi: 10.7554/eLife.100797


    Figure Legend Snippet:

    Techniques Used: Binding Assay, Mutagenesis, Transfection, Construct, CRISPR, Plasmid Preparation, Derivative Assay, Expressing, Control, Sequencing, Recombinant, T7EI Assay, DNA Extraction, DNA Purification, cDNA Synthesis, SYBR Green Assay, Illumina Sequencing, Multiplex Assay, Bicinchoninic Acid Protein Assay, Western Blot, Selection, Software, Knock-Out, Lysis, Electron Microscopy

    Related Articles

    Knock-Out:

    Article Title: Optimization of Buffalo ( Bubalus bubalis ) Embryonic Stem Cell Culture System
    Article Snippet: .. Briefly the inner cell mass from the embryos was dissected out and seeded overmitomycin-C treated buffalo fetal fibroblast cells in ES medium consisting of Knockout Dulbecco’s Modified Eagle Medium (KO-DMEM, GIBCO/BRL) supplemented with 15% knockout serum replacement medium (GIBCO/BRL), 2 mM L-glutamine, 0.1 mM β-mercaptoethanol, 1% nonessential amino acids (all from GIBCO/BRL), 1000 U/ mL LIF, and 5 ng/ mL FGF-2 (R & D Systems). ..

    Article Title: DMSO-Free Programmed Cryopreservation of Fully Dissociated and Adherent Human Induced Pluripotent Stem Cells
    Article Snippet: .. Briefly, cells were cultured in Knockout Dulbecco's modified Eagle's medium (KODMEM, Invitrogen, no. 10829-018) supplemented with 1 mM L-glutamine with 20% Knockout Serum Replacement medium (KOSR, Invitrogen), 1 mM sodium pyruvate, 0.1 mM nonessential amino acids (NEAA, Invitrogen), 50 U/mL penicillin, 50 μ g/mL streptomycin (Invitrogen), 0.1 mM beta-mercaptoethanol (Invitrogen), and 8 ng/mL basic fibroblast growth factor (bFGF, Sigma no. F0291-25UG). .. hESCs and hiPSCs were grown on Matrigel (growth factor-reduced, BD Bioscience) coated 6-well plates (Corning, Inc. no. 3506) on a feeder layer of primary MEFs from E13.5 CD-1 mice.

    Article Title: GATA6 regulates WNT and BMP programs to pattern precardiac mesoderm during the earliest stages of human cardiogenesis
    Article Snippet: .. Other , KnockOut serum replacement medium , Thermo Fisher , 10828010 , Medium. .. Other , RPMI 1640 medium , Thermo Fisher , 2240089 , Medium.

    Article Title: Directed differentiation of human pluripotent stem cells into epidermal keratinocyte-like cells.
    Article Snippet: .. 1 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Mouse anti-cytokeratin 14 Thermo Fisher Scientific MAS-11599 Rabbit anti-cytokeratin 18 Abcam Ab181597 Mouse anti-cytokeratin 19 Chemicon CBL198 Rabbit anti-Involucrin Abcam Ab53112 Rabbiti anti-Loricrin Abcam Ab85679 Rabbit anti -E-Cadherin Cell Signaling 24E10 Rabbit anti-Filaggrin Abcam Ab81468 Rabbit anti-Laminin Abcam Ab14509 Mouse anti-Actin Santa Cruz Sc-47778 Donkey anti-rabbit, Alexa Fluor 488 (1:500 dilution) Invitrogen A21206 Goat anti-mouse, Alexa Fluor 488 (1:500 dilution) Invitrogen A21202 Donkey anti-rabbit, Alexa Fluor 594 (1:500 dilution) Invitrogen A10042 Donkey anti-mouse, Alexa Fluor 594 (1:500 dilution) Invitrogen A10037 Chemicals, peptides, and recombinant proteins DMEM/F12 Thermo Fisher Scientific Cat#1260012 Knockout serum replacement medium Thermo Fisher Scientific Cat#10828010 N2-supplement Thermo Fisher Scientific Cat#17502048 mTeSR1 STEMCELL Technologies Cat#85850 L-Glutamine Thermo Fisher Scientific Cat#25030081 Non-essential amino acids Thermo Fisher Scientific Cat#11140050 b-Mercaptoethanol Thermo Fisher Scientific Cat#21985023 RelesR STEMCELL Technologies Cat#05873 TrypLE Express Enzyme Thermo Fisher Scientific Cat#12604013 Penicillin/Streptomycin Thermo Fisher Scientific Cat#15140152 Retinoic acid Sigma-Aldrich Cat#R2625 BMP4 Thermo Fisher Scientific Cat#PHC9531 EGF Thermo Fisher Scientific Cat#PHG0311 Matrigel hESC-Qualified Matrix Gibco Cat#A14133 Y-27632 dihydrochloride (Rock Inhibitor) Cayman Chemical Cat#10005583 Hoechst 33258 Invitrogen Cat#H3569 CaCl2 Sigma-Aldrich Cat#746495 Paraformaldehyde Santa Cruz Biotechnology Cat#Sc-281692 Bovine serum albumin (BSA) Sigma-Aldrich Cat#A7030 Experimental models: Cell lines hESCs line H1 WiCell Research Institute, Inc. WA01 Ctr1-iPSCs iPSC line generated in our lab from healthy individual QBRIi001-A Ctr2-iPSCs iPSC line generated in our lab from healthy individual QBRIi002-A PsO1-iPSCs-C1 iPSC line generated in our lab from a patient with psoriasis QBRIi005-A Oligonucleotides OCT4: Forward (GACAGGGGGAGGG GAGGAGCTAGG); Reverse (CTTCCCT CCAACCAGTTGCCCCAAAC) Integrated DNA Technologies N/A Nanog: Forward (CATGAGTGTGG ATCCAGCTTG); Reverse (CCTGAAT AAGCAGATCCATGG) Integrated DNA Technologies N/A KRT18: Forward (GTACTGGTCTC AGCAGATTG); Reverse (CTGGCC TTCAGATTTCTCAT) Integrated DNA Technologies N/A P63: Forward (TTCGGACAGTACA AAGAAC); Reverse (CCCTCACTG GTAAGTATAAC) Integrated DNA Technologies N/A (Continued on next page) ll OPEN ACCESS 2 STAR Protocols 3, 101613, September 16, 2022 Protocol Continued REAGENT or RESOURCE SOURCE IDENTIFIER Other 6-well plate Eppendorf Cat#0030720113 12-well plate Eppendorf Cat#0030721110 24-well plate Eppendorf Cat#0030722116 ll OPEN ACCESSProtocol ..

    Article Title: Directed differentiation of human pluripotent stem cells into epidermal keratinocyte-like cells
    Article Snippet: DMEM/F12 , Thermo Fisher Scientific , Cat#1260012. .. Knockout serum replacement medium , Thermo Fisher Scientific , Cat#10828010. .. N2-supplement , Thermo Fisher Scientific , Cat#17502048.

    Article Title: Activation and Inhibition of The Wnt3A Signaling Pathway in Buffalo ( Bubalus bubalis ) Embryonic Stem Cells: Effects of WNT3A, Bio and Dkk1
    Article Snippet: .. Briefly, we mechanically dissected the inner cell mass from the embryos under a zoom stereomicroscope and seeded them onto a mitomycintreated buffalo fetal fibroblast feeder layer in ES medium that consisted of knockout Dulbecco’s modified eagle medium ( KO-DMEM, Gibco/ BRL ) supplemented with 15% knockout serum replacement medium ( Gibco/BRL ), 2 mM L-glutamine, 0.1 mM β-mercaptoethanol, 1% nonessential amino acids ( all from Gibco/BRL ), 1000 U/ mL LIF, and 5 ng/mL FGF-2 ( R & D Systems ). ..

    Article Title: Neural progenitor cells derived from fibroblasts induced by small molecule compounds under hypoxia for treatment of Parkinson's disease in rats.
    Article Snippet: Rat NSCs were derived from E16 mouse embryos and expanded in a neural expansion medium (NEM; NeuroCult Basal medium, STEMCELL Technologies, Vancouver, BC, Canada), supplemented with 2% B27, 20 ng/mL epidermal growth factor (EGF), and 20 ng/mL basic fibroblast growth factor (bFGF). .. To chemically induce the conversion of REFs into ciNPCs, the initial REFs at P3 cultured in H-DMEM supplemented with 10% fetal bovine serum for 48 hours were transferred into a knockout serum replacement medium (Thermo Fisher Scientific), which contained knockout Dulbecco’s modified Eagle’s medium (DMEM), 15% knockout serum replacement, 1% non-essential amino acids, 1% GlutaMax, 1% sodium pyruvate, 0.1 mM β-mercaptoethanol, 1000 U/mL leukemia inhibitory factor, 0.5 mM valproic acid (Sigma-Aldrich, Merck KGaA, Darmstadt, Germany), 3 μM CHIR99021 (Sigma-Aldrich), and 1 μM Repsox (Selleckchem, Houston, TX, USA). ..

    Article Title: Lentiviral Vectors and Protocols for Creation of Stable hESC Lines for Fluorescent Tracking and Drug Resistance Selection of Cardiomyocytes
    Article Snippet: .. Briefly, cells were cultured in Knockout Dulbecco's modified Eagle's medium (KODMEM, Invitrogen, 10829-018) supplemented with 1 mM L-glutamine with 20% Knockout Serum Replacement medium (KOSR, Invitrogen), 1 mM sodium pyruvate, 0.1 mM nonessential amino acids (NEAA, Invitrogen), 50 U/ml penicillin, 50 μg/ml streptomycin (Invitrogen), 0.1 mM beta-mercaptoethanol (Invitrogen) and 8 ng/ml basic fibroblast growth factor (bFGF, Sigma catalogue F0291-25UG). hESCs were grown on Matrigel (growth factor-reduced, BD Bioscience)-coated 6-well plates (Corning, Inc. catalogue 3506) on a feeder layer of primary MEFs from E13.5 CD-1 mice isolated as described . ..

    Modification:

    Article Title: Optimization of Buffalo ( Bubalus bubalis ) Embryonic Stem Cell Culture System
    Article Snippet: .. Briefly the inner cell mass from the embryos was dissected out and seeded overmitomycin-C treated buffalo fetal fibroblast cells in ES medium consisting of Knockout Dulbecco’s Modified Eagle Medium (KO-DMEM, GIBCO/BRL) supplemented with 15% knockout serum replacement medium (GIBCO/BRL), 2 mM L-glutamine, 0.1 mM β-mercaptoethanol, 1% nonessential amino acids (all from GIBCO/BRL), 1000 U/ mL LIF, and 5 ng/ mL FGF-2 (R & D Systems). ..

    Article Title: DMSO-Free Programmed Cryopreservation of Fully Dissociated and Adherent Human Induced Pluripotent Stem Cells
    Article Snippet: .. Briefly, cells were cultured in Knockout Dulbecco's modified Eagle's medium (KODMEM, Invitrogen, no. 10829-018) supplemented with 1 mM L-glutamine with 20% Knockout Serum Replacement medium (KOSR, Invitrogen), 1 mM sodium pyruvate, 0.1 mM nonessential amino acids (NEAA, Invitrogen), 50 U/mL penicillin, 50 μ g/mL streptomycin (Invitrogen), 0.1 mM beta-mercaptoethanol (Invitrogen), and 8 ng/mL basic fibroblast growth factor (bFGF, Sigma no. F0291-25UG). .. hESCs and hiPSCs were grown on Matrigel (growth factor-reduced, BD Bioscience) coated 6-well plates (Corning, Inc. no. 3506) on a feeder layer of primary MEFs from E13.5 CD-1 mice.

    Article Title: Activation and Inhibition of The Wnt3A Signaling Pathway in Buffalo ( Bubalus bubalis ) Embryonic Stem Cells: Effects of WNT3A, Bio and Dkk1
    Article Snippet: .. Briefly, we mechanically dissected the inner cell mass from the embryos under a zoom stereomicroscope and seeded them onto a mitomycintreated buffalo fetal fibroblast feeder layer in ES medium that consisted of knockout Dulbecco’s modified eagle medium ( KO-DMEM, Gibco/ BRL ) supplemented with 15% knockout serum replacement medium ( Gibco/BRL ), 2 mM L-glutamine, 0.1 mM β-mercaptoethanol, 1% nonessential amino acids ( all from Gibco/BRL ), 1000 U/ mL LIF, and 5 ng/mL FGF-2 ( R & D Systems ). ..

    Article Title: Neural progenitor cells derived from fibroblasts induced by small molecule compounds under hypoxia for treatment of Parkinson's disease in rats.
    Article Snippet: Rat NSCs were derived from E16 mouse embryos and expanded in a neural expansion medium (NEM; NeuroCult Basal medium, STEMCELL Technologies, Vancouver, BC, Canada), supplemented with 2% B27, 20 ng/mL epidermal growth factor (EGF), and 20 ng/mL basic fibroblast growth factor (bFGF). .. To chemically induce the conversion of REFs into ciNPCs, the initial REFs at P3 cultured in H-DMEM supplemented with 10% fetal bovine serum for 48 hours were transferred into a knockout serum replacement medium (Thermo Fisher Scientific), which contained knockout Dulbecco’s modified Eagle’s medium (DMEM), 15% knockout serum replacement, 1% non-essential amino acids, 1% GlutaMax, 1% sodium pyruvate, 0.1 mM β-mercaptoethanol, 1000 U/mL leukemia inhibitory factor, 0.5 mM valproic acid (Sigma-Aldrich, Merck KGaA, Darmstadt, Germany), 3 μM CHIR99021 (Sigma-Aldrich), and 1 μM Repsox (Selleckchem, Houston, TX, USA). ..

    Article Title: Lentiviral Vectors and Protocols for Creation of Stable hESC Lines for Fluorescent Tracking and Drug Resistance Selection of Cardiomyocytes
    Article Snippet: .. Briefly, cells were cultured in Knockout Dulbecco's modified Eagle's medium (KODMEM, Invitrogen, 10829-018) supplemented with 1 mM L-glutamine with 20% Knockout Serum Replacement medium (KOSR, Invitrogen), 1 mM sodium pyruvate, 0.1 mM nonessential amino acids (NEAA, Invitrogen), 50 U/ml penicillin, 50 μg/ml streptomycin (Invitrogen), 0.1 mM beta-mercaptoethanol (Invitrogen) and 8 ng/ml basic fibroblast growth factor (bFGF, Sigma catalogue F0291-25UG). hESCs were grown on Matrigel (growth factor-reduced, BD Bioscience)-coated 6-well plates (Corning, Inc. catalogue 3506) on a feeder layer of primary MEFs from E13.5 CD-1 mice isolated as described . ..

    Gene Knockout:

    Article Title: Optimization of Buffalo ( Bubalus bubalis ) Embryonic Stem Cell Culture System
    Article Snippet: .. Briefly the inner cell mass from the embryos was dissected out and seeded overmitomycin-C treated buffalo fetal fibroblast cells in ES medium consisting of Knockout Dulbecco’s Modified Eagle Medium (KO-DMEM, GIBCO/BRL) supplemented with 15% knockout serum replacement medium (GIBCO/BRL), 2 mM L-glutamine, 0.1 mM β-mercaptoethanol, 1% nonessential amino acids (all from GIBCO/BRL), 1000 U/ mL LIF, and 5 ng/ mL FGF-2 (R & D Systems). ..

    Article Title: Activation and Inhibition of The Wnt3A Signaling Pathway in Buffalo ( Bubalus bubalis ) Embryonic Stem Cells: Effects of WNT3A, Bio and Dkk1
    Article Snippet: .. Briefly, we mechanically dissected the inner cell mass from the embryos under a zoom stereomicroscope and seeded them onto a mitomycintreated buffalo fetal fibroblast feeder layer in ES medium that consisted of knockout Dulbecco’s modified eagle medium ( KO-DMEM, Gibco/ BRL ) supplemented with 15% knockout serum replacement medium ( Gibco/BRL ), 2 mM L-glutamine, 0.1 mM β-mercaptoethanol, 1% nonessential amino acids ( all from Gibco/BRL ), 1000 U/ mL LIF, and 5 ng/mL FGF-2 ( R & D Systems ). ..

    Cell Culture:

    Article Title: DMSO-Free Programmed Cryopreservation of Fully Dissociated and Adherent Human Induced Pluripotent Stem Cells
    Article Snippet: .. Briefly, cells were cultured in Knockout Dulbecco's modified Eagle's medium (KODMEM, Invitrogen, no. 10829-018) supplemented with 1 mM L-glutamine with 20% Knockout Serum Replacement medium (KOSR, Invitrogen), 1 mM sodium pyruvate, 0.1 mM nonessential amino acids (NEAA, Invitrogen), 50 U/mL penicillin, 50 μ g/mL streptomycin (Invitrogen), 0.1 mM beta-mercaptoethanol (Invitrogen), and 8 ng/mL basic fibroblast growth factor (bFGF, Sigma no. F0291-25UG). .. hESCs and hiPSCs were grown on Matrigel (growth factor-reduced, BD Bioscience) coated 6-well plates (Corning, Inc. no. 3506) on a feeder layer of primary MEFs from E13.5 CD-1 mice.

    Article Title: Neural progenitor cells derived from fibroblasts induced by small molecule compounds under hypoxia for treatment of Parkinson's disease in rats.
    Article Snippet: Rat NSCs were derived from E16 mouse embryos and expanded in a neural expansion medium (NEM; NeuroCult Basal medium, STEMCELL Technologies, Vancouver, BC, Canada), supplemented with 2% B27, 20 ng/mL epidermal growth factor (EGF), and 20 ng/mL basic fibroblast growth factor (bFGF). .. To chemically induce the conversion of REFs into ciNPCs, the initial REFs at P3 cultured in H-DMEM supplemented with 10% fetal bovine serum for 48 hours were transferred into a knockout serum replacement medium (Thermo Fisher Scientific), which contained knockout Dulbecco’s modified Eagle’s medium (DMEM), 15% knockout serum replacement, 1% non-essential amino acids, 1% GlutaMax, 1% sodium pyruvate, 0.1 mM β-mercaptoethanol, 1000 U/mL leukemia inhibitory factor, 0.5 mM valproic acid (Sigma-Aldrich, Merck KGaA, Darmstadt, Germany), 3 μM CHIR99021 (Sigma-Aldrich), and 1 μM Repsox (Selleckchem, Houston, TX, USA). ..

    Article Title: Lentiviral Vectors and Protocols for Creation of Stable hESC Lines for Fluorescent Tracking and Drug Resistance Selection of Cardiomyocytes
    Article Snippet: .. Briefly, cells were cultured in Knockout Dulbecco's modified Eagle's medium (KODMEM, Invitrogen, 10829-018) supplemented with 1 mM L-glutamine with 20% Knockout Serum Replacement medium (KOSR, Invitrogen), 1 mM sodium pyruvate, 0.1 mM nonessential amino acids (NEAA, Invitrogen), 50 U/ml penicillin, 50 μg/ml streptomycin (Invitrogen), 0.1 mM beta-mercaptoethanol (Invitrogen) and 8 ng/ml basic fibroblast growth factor (bFGF, Sigma catalogue F0291-25UG). hESCs were grown on Matrigel (growth factor-reduced, BD Bioscience)-coated 6-well plates (Corning, Inc. catalogue 3506) on a feeder layer of primary MEFs from E13.5 CD-1 mice isolated as described . ..

    Recombinant:

    Article Title: Directed differentiation of human pluripotent stem cells into epidermal keratinocyte-like cells.
    Article Snippet: .. 1 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Mouse anti-cytokeratin 14 Thermo Fisher Scientific MAS-11599 Rabbit anti-cytokeratin 18 Abcam Ab181597 Mouse anti-cytokeratin 19 Chemicon CBL198 Rabbit anti-Involucrin Abcam Ab53112 Rabbiti anti-Loricrin Abcam Ab85679 Rabbit anti -E-Cadherin Cell Signaling 24E10 Rabbit anti-Filaggrin Abcam Ab81468 Rabbit anti-Laminin Abcam Ab14509 Mouse anti-Actin Santa Cruz Sc-47778 Donkey anti-rabbit, Alexa Fluor 488 (1:500 dilution) Invitrogen A21206 Goat anti-mouse, Alexa Fluor 488 (1:500 dilution) Invitrogen A21202 Donkey anti-rabbit, Alexa Fluor 594 (1:500 dilution) Invitrogen A10042 Donkey anti-mouse, Alexa Fluor 594 (1:500 dilution) Invitrogen A10037 Chemicals, peptides, and recombinant proteins DMEM/F12 Thermo Fisher Scientific Cat#1260012 Knockout serum replacement medium Thermo Fisher Scientific Cat#10828010 N2-supplement Thermo Fisher Scientific Cat#17502048 mTeSR1 STEMCELL Technologies Cat#85850 L-Glutamine Thermo Fisher Scientific Cat#25030081 Non-essential amino acids Thermo Fisher Scientific Cat#11140050 b-Mercaptoethanol Thermo Fisher Scientific Cat#21985023 RelesR STEMCELL Technologies Cat#05873 TrypLE Express Enzyme Thermo Fisher Scientific Cat#12604013 Penicillin/Streptomycin Thermo Fisher Scientific Cat#15140152 Retinoic acid Sigma-Aldrich Cat#R2625 BMP4 Thermo Fisher Scientific Cat#PHC9531 EGF Thermo Fisher Scientific Cat#PHG0311 Matrigel hESC-Qualified Matrix Gibco Cat#A14133 Y-27632 dihydrochloride (Rock Inhibitor) Cayman Chemical Cat#10005583 Hoechst 33258 Invitrogen Cat#H3569 CaCl2 Sigma-Aldrich Cat#746495 Paraformaldehyde Santa Cruz Biotechnology Cat#Sc-281692 Bovine serum albumin (BSA) Sigma-Aldrich Cat#A7030 Experimental models: Cell lines hESCs line H1 WiCell Research Institute, Inc. WA01 Ctr1-iPSCs iPSC line generated in our lab from healthy individual QBRIi001-A Ctr2-iPSCs iPSC line generated in our lab from healthy individual QBRIi002-A PsO1-iPSCs-C1 iPSC line generated in our lab from a patient with psoriasis QBRIi005-A Oligonucleotides OCT4: Forward (GACAGGGGGAGGG GAGGAGCTAGG); Reverse (CTTCCCT CCAACCAGTTGCCCCAAAC) Integrated DNA Technologies N/A Nanog: Forward (CATGAGTGTGG ATCCAGCTTG); Reverse (CCTGAAT AAGCAGATCCATGG) Integrated DNA Technologies N/A KRT18: Forward (GTACTGGTCTC AGCAGATTG); Reverse (CTGGCC TTCAGATTTCTCAT) Integrated DNA Technologies N/A P63: Forward (TTCGGACAGTACA AAGAAC); Reverse (CCCTCACTG GTAAGTATAAC) Integrated DNA Technologies N/A (Continued on next page) ll OPEN ACCESS 2 STAR Protocols 3, 101613, September 16, 2022 Protocol Continued REAGENT or RESOURCE SOURCE IDENTIFIER Other 6-well plate Eppendorf Cat#0030720113 12-well plate Eppendorf Cat#0030721110 24-well plate Eppendorf Cat#0030722116 ll OPEN ACCESSProtocol ..

    Generated:

    Article Title: Directed differentiation of human pluripotent stem cells into epidermal keratinocyte-like cells.
    Article Snippet: .. 1 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Mouse anti-cytokeratin 14 Thermo Fisher Scientific MAS-11599 Rabbit anti-cytokeratin 18 Abcam Ab181597 Mouse anti-cytokeratin 19 Chemicon CBL198 Rabbit anti-Involucrin Abcam Ab53112 Rabbiti anti-Loricrin Abcam Ab85679 Rabbit anti -E-Cadherin Cell Signaling 24E10 Rabbit anti-Filaggrin Abcam Ab81468 Rabbit anti-Laminin Abcam Ab14509 Mouse anti-Actin Santa Cruz Sc-47778 Donkey anti-rabbit, Alexa Fluor 488 (1:500 dilution) Invitrogen A21206 Goat anti-mouse, Alexa Fluor 488 (1:500 dilution) Invitrogen A21202 Donkey anti-rabbit, Alexa Fluor 594 (1:500 dilution) Invitrogen A10042 Donkey anti-mouse, Alexa Fluor 594 (1:500 dilution) Invitrogen A10037 Chemicals, peptides, and recombinant proteins DMEM/F12 Thermo Fisher Scientific Cat#1260012 Knockout serum replacement medium Thermo Fisher Scientific Cat#10828010 N2-supplement Thermo Fisher Scientific Cat#17502048 mTeSR1 STEMCELL Technologies Cat#85850 L-Glutamine Thermo Fisher Scientific Cat#25030081 Non-essential amino acids Thermo Fisher Scientific Cat#11140050 b-Mercaptoethanol Thermo Fisher Scientific Cat#21985023 RelesR STEMCELL Technologies Cat#05873 TrypLE Express Enzyme Thermo Fisher Scientific Cat#12604013 Penicillin/Streptomycin Thermo Fisher Scientific Cat#15140152 Retinoic acid Sigma-Aldrich Cat#R2625 BMP4 Thermo Fisher Scientific Cat#PHC9531 EGF Thermo Fisher Scientific Cat#PHG0311 Matrigel hESC-Qualified Matrix Gibco Cat#A14133 Y-27632 dihydrochloride (Rock Inhibitor) Cayman Chemical Cat#10005583 Hoechst 33258 Invitrogen Cat#H3569 CaCl2 Sigma-Aldrich Cat#746495 Paraformaldehyde Santa Cruz Biotechnology Cat#Sc-281692 Bovine serum albumin (BSA) Sigma-Aldrich Cat#A7030 Experimental models: Cell lines hESCs line H1 WiCell Research Institute, Inc. WA01 Ctr1-iPSCs iPSC line generated in our lab from healthy individual QBRIi001-A Ctr2-iPSCs iPSC line generated in our lab from healthy individual QBRIi002-A PsO1-iPSCs-C1 iPSC line generated in our lab from a patient with psoriasis QBRIi005-A Oligonucleotides OCT4: Forward (GACAGGGGGAGGG GAGGAGCTAGG); Reverse (CTTCCCT CCAACCAGTTGCCCCAAAC) Integrated DNA Technologies N/A Nanog: Forward (CATGAGTGTGG ATCCAGCTTG); Reverse (CCTGAAT AAGCAGATCCATGG) Integrated DNA Technologies N/A KRT18: Forward (GTACTGGTCTC AGCAGATTG); Reverse (CTGGCC TTCAGATTTCTCAT) Integrated DNA Technologies N/A P63: Forward (TTCGGACAGTACA AAGAAC); Reverse (CCCTCACTG GTAAGTATAAC) Integrated DNA Technologies N/A (Continued on next page) ll OPEN ACCESS 2 STAR Protocols 3, 101613, September 16, 2022 Protocol Continued REAGENT or RESOURCE SOURCE IDENTIFIER Other 6-well plate Eppendorf Cat#0030720113 12-well plate Eppendorf Cat#0030721110 24-well plate Eppendorf Cat#0030722116 ll OPEN ACCESSProtocol ..

    Isolation:

    Article Title: Lentiviral Vectors and Protocols for Creation of Stable hESC Lines for Fluorescent Tracking and Drug Resistance Selection of Cardiomyocytes
    Article Snippet: .. Briefly, cells were cultured in Knockout Dulbecco's modified Eagle's medium (KODMEM, Invitrogen, 10829-018) supplemented with 1 mM L-glutamine with 20% Knockout Serum Replacement medium (KOSR, Invitrogen), 1 mM sodium pyruvate, 0.1 mM nonessential amino acids (NEAA, Invitrogen), 50 U/ml penicillin, 50 μg/ml streptomycin (Invitrogen), 0.1 mM beta-mercaptoethanol (Invitrogen) and 8 ng/ml basic fibroblast growth factor (bFGF, Sigma catalogue F0291-25UG). hESCs were grown on Matrigel (growth factor-reduced, BD Bioscience)-coated 6-well plates (Corning, Inc. catalogue 3506) on a feeder layer of primary MEFs from E13.5 CD-1 mice isolated as described . ..



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    Image Search Results


    Journal: eLife

    Article Title: GATA6 regulates WNT and BMP programs to pattern precardiac mesoderm during the earliest stages of human cardiogenesis

    doi: 10.7554/eLife.100797

    Figure Lengend Snippet:

    Article Snippet: Other , KnockOut serum replacement medium , Thermo Fisher , 10828010 , Medium.

    Techniques: Binding Assay, Mutagenesis, Transfection, Construct, CRISPR, Plasmid Preparation, Derivative Assay, Expressing, Control, Sequencing, Recombinant, T7EI Assay, DNA Extraction, DNA Purification, cDNA Synthesis, SYBR Green Assay, Illumina Sequencing, Multiplex Assay, Bicinchoninic Acid Protein Assay, Western Blot, Selection, Software, Knock-Out, Lysis, Electron Microscopy